Investigation of C609T polymorphism in the NQO1 gene in patients diagnosed with colorectal cancer in the Azerbaijani population

Research article: Investigation of C609T polymorphism in the NQO1 gene in patients diagnosed with colorectal cancer in the Azerbaijani population

Authors: Bayramov Bayram Ilham1*, Bayramov Nuru Yusif2

1 Genetic Resources Institute, Azerbaijan National Academy of Sciences, 155 Azadlig Ave., Baku AZ1106, Azerbaijan

2 Azerbaijan Educational-Surgical Clinic of Azerbaijan Medical University, 23 A. Bakikhanov Str., Baku AZ1022, Azerbaijan

*For correspondence:

Received: May 02, 2022; Reviewed: May 30, 2021; Accepted: June 12, 2022

Abstract: This study aimed to determine the clinical significance of the NQO1 C609T (rs1800566, Pro187Ser) polymorphism in patients with colorectal cancer (CRC) in the Azerbaijani population. 142 patients with CRC and 146 healthy individuals were included in the study. Manual DNA was isolated from blood using the salting-out method, and genotypes were determined on agarose gel using the PCR-RFLP method. When comparing the patients and control groups, heterozygous CT (OR=1.813; 95%CI=1.097–2.995, P=0.020), under the dominant model (OR=1.842; 95%CI=1.137–2.983, P=0.013), and the mutant T allele (OR=1.644; 95%CI=1.096–2.465, P=0.016) were statistically associated with an increased risk of CRC. When comparing male patients with healthy men, heterozygous CT was associated with a higher risk of CRC (OR=2.219; 95%CI=1.079-4.565, P=0.029). However, the age, pathological tumor grade and stage, smoking, and alcohol consumption of the study groups were compared and no significant relationship was found (P>0.05). Our findings showed that the NQO1 C609T polymorphism is related to the risk of colorectal cancer in the Azerbaijan population.

Keywords: Colorectal cancer, polymorphism, PCR-RFLP, NQO1, C609T


Biller L.H., Schrag D. (2021) Diagnosis and treatment of metastatic colorectal cancer: A review. JAMA, 325(7): 669-685. 

Ding R., Lin S., Chen D. (2012).. Association of NQO1 rs1800566 polymorphism and the risk of colorectal cancer: a meta-analysis. Int J Colorectal Dis., 27(7): 885-892. 

Holme Ø., Bretthauer M., Fretheim A., Odgaard-Jensen J., Hoff G. (2013).. Flexible sigmoidoscopy versus faecal occult blood testing for colorectal cancer screening in asymptomatic individuals. Cochrane Database Syst Rev., (9): CD009259. 

Iskander K., Jaiswal A.K. (2005) Quinone oxidoreductases in protection against myelogenous hyperplasia and benzene toxicity. Chem Biol Interact., 153-154: 147-157. 

Li G.M. (2008) Mechanisms and functions of DNA mismatch repair. Cell Res., 18: 85-98; doi: 10.1038/cr.115 

Nebert D.W., Roe A.L., Vandale S.E., Bingham E., Oakley G.G. (2002) NAD(P)H: quinoneoxidoreductase (NQO1) polymorphism, exposure to benzene, and predisposition to disease: a HuGE review. Genet Med., 4: 62-70. 

Nguyen L.H., Goel A., Chung D.C. (2020) Pathways of colorectal carcinogenesis. Gastroenterology, 158: 291–302. 

Peng X.E., Jiang Y.Y., Shi X.S., Hu Z.J. (2013) NQO1 609C>T polymorphism interaction with tobacco smoking and alcohol drinking increases colorectal cancer risk in a Chinese population. Gene, 521(1): 105-110. 

Sameer A.S., Shah Z.A., Syeed N., Rasool R., Afroze D., Siddiqi M.A. (2010) NAD(P)H:quinone oxidoreductase 1 (NQO1) Pro187Ser polymorphism and colorectal cancer predisposition in the ethnic Kashmiri population. Asian Pac. J. Cancer Prev., 11(1): 209-213. 

Siegel R.L., Miller K.D., Jemal A. (2018) Cancer statistics, CA. Cancer J. Clin., 68: 7–30. 

Wang Y., Zhang G., Luo Y. (2013) Association between NQO1 C609T polymorphism and colorectal cancer risk. Tumour Biol., 34(6): 4027-4032. 

Welch H.G., Robertson D.J. (2016) Colorectal cancer on the decline - why screening can't explain it all. N. Engl. J. Med., 374(17): 1605-1607. 

Yadav U., Kumar P., Rai V. (2018) NQO1 gene C609T polymorphism (dbSNP: rs1800566) and digestive tract cancer risk: A meta-analysis. Nutr. Cancer., 70(4): 557-568. 

Zhao H., Zhu F., Sun J., Meng X. (2014) Meta-analysis of the association between NQO1 Pro187Ser polymorphism and colorectal cancer in Asians. Tumour Biol., 35(3): 2111-2116. 

Zheng B., Wang Z., Chai R. (2014) NQO1 C609T polymorphism and colorectal cancer susceptibility: a meta-analysis. Arch. Med. Sci., 10(4): 651-660.

Добавить комментарий

Оставить комментарий

reload, if the code cannot be seen