Investigation of the interaction of polyene antibiotics with cholesterol

Research article:  Investigation of the interaction of polyene antibiotics with cholesterol
Author (s): T.C. Pashazade, X.M. Gasimov1,2*

1Azerbaijan State Academy of Physical Education and Sport, 98 Fatali Khan Khoyski Ave.,

Baku AZ1072, Azerbaijan

2Institute of Botany, Azerbaijan National of Sciences, 40 Badamdar Highway, Baku AZ1004, Azerbaijan

For correspondence: khalil.gasimov@gmail.com

Received:  April 11, 2021; Received in revised form: May 12, 2021; Accepted: June 09, 2021

Abstract

One of the compounds affected by membranes is polyene antibiotics. Amphotericin B, nystatin, mycoheptin, and levorin are mainly classified in the polyene class and distinguished by high biological activity. The high sensitivity of polyene antibiotics to membranes is due to the cholesterol they contain. The main feature of polyene antibiotics is the formation of structural ion channels by combining with cholesterol in the membranes. The interaction of polyene antibiotics with cholesterol has been demonstrated by obtaining ultraviolet (UV) spectra. Polyenes have three primary absorption spectra and range from 370 nm to 430 nm. Amphotericin B and levorin complex with cholesterol reduce the maximum amplitude of UV absorption spectra. The results show that cholesterol molecules combine with the double-bond chain systems of amphotericin B and levorin to reduce the maximum amplitude of UV absorption spectra. UV spectrum of dimethyl-sulfoxide molecules has been obtained. Its absorption spectrum ranges from 240 nm to 250 nm. The absorption spectrum of dimethyl sulfoxide molecules at these waves is related to the presence of the disulfide S=O group.

Keywords: Levorin, amphotericin B, cholesterol, ultraviolet spectrum, cholesterol-polyene complex

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