Studies of underlying molecular mechanisms of retinitis pigmentosa in experimental model and clinics
Research article: Studies of underlying molecular mechanisms of retinitis pigmentosa in experimental model and clinics
Author: U.S. Ismailova
Academician Abdulla Garayev Institute of Physiology, Azerbaijan National Academy of Sciences, 78 Sharifzadeh Str., Baku AZ1100, Azerbaijan
For correspondence: arifmekht@yahoo.com
Accepted for publication: 12 June 2020
Abstract:
The article concerns the analysis of underlying molecular mechanisms of retinitis pigmentosa on the experimental model of the rabbits and on the patients diagnosed with this pathology. The studi- es were conducted on Chinchilla male rabbits having body mass of 2.2-2.6 kg. Serum was obtained from the patients with retinitis pigmentosa. Retinitis pigmentosa was modelled in the rabbits thro- ugh i.v. administration of monoiodacetic acid (MIAA; grave degree, 26 mg/kg of body mass). With the application of indirect ELISA-test the levels of serotonin-modulating anticonsolidation protein (SMAP; Mekhtiev, 2000) in the hypothalamus, heat shock protein 70 kDa (HSP70) and rhodopsin in the retina and natural anti-SMAP autoantibodies in the serum of the patients were measured. The data were analysed on Student’s t-criterion. Significant downregulation of rhodopsin (p<0.001) and upregulation of HSP70 (p<0.001) in the retina, as well as upregulation of SMAP (p<0.01) in the hypothalamus of the MIAA-administered rabbits were noticed. Intra-vitreal administration of SMAP to the MIAA-administered rabbits resulted in a significant upregulation of rhodopsin (p<0.001) and HSP70 (p<0.001) in the retina. Noticeable downregulation of the titres of natural autoimmune anti-SMAP antibodies in the serum of the diagnosed patients with retinitis pigmentosa relatively to healthy persons of the same age (p<0.01) was revealed. Molecular mechanisms underl- ying hypothalamic trophic regulatory effects on retina receptor cells through retrograde and ante- rograde axonal transports are considered.
Keywords: Retinitis pigmentosa, rabbits, serotonin-modulating anticonsolidation protein (SMAP), rho- dopsin, heat-shock protein 70, anti-SMAP antibodies, retina, hypothalamus, natural anti-SMAP autoanti- bodies.
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