Transgenerational transmission of prenatal hypoxia-induced changes of two enzymes in the brain structures of rat progeny
Research article: Transgenerational transmission of prenatal hypoxia-induced changes of two enzymes in the brain structures of rat progeny
Authors: E.Sh. Abiyeva, A.A. Mekhtiev*
Academician Abdulla Garayev Institute of Physiology, Azerbaijan National Academy of Sciences, 78 Sharifzadeh Str., Baku AZ1100, Azerbaijan;
* For correspondence: arifmekht@yahoo.com
Accepted for publication: 12 March 2020
Abstract:
The article concerns the problem of transgenerational transmission of the changed activities of the enzymes of pyrophosphatase and succinate dehydrogenase in the rats. The experiments were carri- ed out on 6-month-old female Wistar rats and their progeny at the age of 17 and 30 days. The gesta- ted rats, whose fetus were at the stage of organogenesis, were subjected to 20-minute daily hypoxia (gas mixture of 90% nitrogen and 10% oxygen) for 5 days. The rat pups born from these females, were decapitated and fragments of the orbital, sensorimotor and limbic cortex, hypothalamus and cerebellum were taken from their brains and mitochondrial and cytosol fractions were fractiona- ted. In the mitochondrial fractions of the all studied structures, significant downregulation of the pyrophosphatase activity was found in the 17-day-old rat pups, though in the cytosol fraction it was noted only in the orbital cortex. In the mitochondrial (except for the orbital and limbic cortex) and cytosol fractions of the all studied structures of the 30-day-old rats, downregulation of pyrophosp- hatase as well was observed. On the contrary, in the 17-day-old rat pups in the mitochondrial frac- tions of the all studied structures, prominent upregulation of the activity of succinate dehydrogena- se with its simultaneous downregulation in the cytosol fractions of the orbital and limbic cortex, and cerebellum was revealed. In the 30-day-old rat pups, significant upregulation of this enzyme activity was observed in the cytosol fractions of the all studied structures, whereas in the mitoc- hondrial fractions (except for sensorimotor cortex) no changes were noted. It is concluded that a transgenerational transmission of the altered activity of two enzymes occur, apparently due to epi- genetic changes in the activity of the corresponding genes.
Keywords: Pyrophosphatase, succinate dehydrogenase, rats, brain structures, transgenerational trans- mission.
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