Thermophilic bacteria of the hot springs “Ashagi Istisu” and “Yukhari Istisu” of the Kalbajar region of the Republic of Azerbaijan G.Y. Gunashova*, F.R. Ahmadova, R.I. Khalilov

Research article: Thermophilic bacteria of the hot springs “Ashagi   Istisu” and “Yukhari Istisu” of the Kalbajar region of the Republic of Azerbaijan

Author (s): G.Y. Gunashova*, F.R. Ahmadova, R.I. Khalilov

DOI: http://dx.doi.org/10.29228/jlsb.33  

Baku State University, 23 Academician Z.Khalilov Str., Baku AZ1148, Azerbaijan 

*For correspondence:  gunay_a-va1995@mail.ru 

Received: September 21, 2021; Received in revised form: September 27, 2021; Accepted: October 22, 2021

ABSTRACT
The presented work is devoted to the study of the bacterial biota of the hot springs “Ashagi Istisu” (pH=8.0, t=64°C, mineralization is 6.7 g/l)  and “Yukhari Istisu” (pH=9.0,  t=71°C, mineralization is 4.3 g/l) of the Kalbajar region, characterized by a wide range of environmental conditions (pH, temperatures, chemical components, etc.). Samples of thermal waters were inoculated and colonies of thermophilic bacteria that grew on a solid medium of nutrient agar were isolated. Some colonies of thermophilic bacteria were isolated in pure culture and 6 strains were received (KA₁, KA₂, KA₃, KY₁, KY₂, KY₃). The cell morphology of these strains is represented by short and long gram-positive, spore - forming rods, varying in size. Colonies differ in color, shape, margin, consistency, size, etc. The morphology of cells and colonies of two strains (KA₁, KY₁) has been well studied, and the ratio of these strains to the pH and temperature of the medium was determined. It was found that strains KA₁, KY₁ actively grow in the temperature range 55-60°C, at pH=7.0-9.0. Based on morpho-cultural characteristics and some physiological properties, the strains were identified. The KA₁ strain was assigned to the genus Bacillus, and the KY₁ strain was presumably assigned to the Bacillus stearothermophilus species.

Keywords: Hot springs, thermophilic bacteria, morpho-cultural characteristics

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