Study of the activity of serotonin-modulating anticonsolidation protein in regulation of proliferation and differentiation of embryonic cells of Xenopus laevis
Research article: Study of the activity of serotonin-modulating anticonsolidation protein in regulation of proliferation and differentiation of embryonic cells of Xenopus laevis
Authors: A.V. Aminov, A.A. Mekhtiev*
Academician Abdulla Garayev Institute of Physiology, Azerbaijan National Academy of Sciences, 78 Sharifzadeh Str., Baku AZ1100, Azerbaijan;
* For correspondence: arifmekht@yahoo.com
Accepted for publication: 15 May 2020
Abstract:
The article concerns the studies of the activity of serotonin-modulating anticonsolidation protein (SMAP), being in linear relations with serotonin, in regulation of cell proliferation and differentiation on the embryonic model of Xenopus laevis. In the 1st series of studies the dynamics of SMAP level in the embryos of Xenopus laevis throughout the all stages of embryogenesis and metamorphosis was pursued with application of indirect ELISA-test. Beginning from the blastula stage till the end of the neurula its level remained unchanged. On the following stages continuous downregulation of the SMAP level, interrupted as a slight upregulation between the 37th stage and onset of the 39th stage, was observed. In the 2nd series of studies, incubation of the embryos of Xenopus laevis on the blastula and gastrula stages in fresh water containing SMAP at a dose of 50 and 100 μg/ml led to delay in de- velopment and, finally, to death of all the embryos within 4 days of the observation. In the 3rd series of the studies, blocking of SMAP activity with the anti-SMAP polyclonal antibodies at a dose of 50 μg/ml in the embryos of Xenopus laevis, being on the 37th stage of development, resulted in their accelerated passing (by two stages earlier) of the metamorphosis stage relative to the intact and control groups. Hence, if on the initial stages of embryogenesis SMAP demonstrates its cytostatic activity, on the me- tamorphosis stage antibodies-mediated blockade of its activity, conversely, leads to accelerated pas- sing of embryonic cells through cell differentiation.
Keywords: Serotonin-modulating anticonsolidation protein, antibodies, embryogenesis, metamorphosis, Xenopus laevis
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