Soft tissues regional blood flow and microvasculature upon different perioperative treatment strategies following indirect revascularization in patients with critical lower limb ischemia, caused by occlusion of distal arteries
Research article: Soft tissues regional blood flow and microvasculature upon different perioperative treatment strategies following indirect revascularization in patients with critical lower limb ischemia, caused by occlusion of distal arteries
Authors: J.V. Kosayev1*, I.A. Hasanov2*
1 Scientific Center of Surgery named after Academician Mustafa Topchubashev, 196 Sharifzadeh Str., Baku AZ1122 Azerbaijan;
2 National Center of Oncology, 79B Zardabi Ave., Baku AZ1012, Azerbaijan;
*For correspondence: email@example.com; firstname.lastname@example.org;
Accepted for publication: 19 June 2020
This work was aimed at studying the effect of different perioperative treatment strategies following indirect revascularization on regional blood flow and microcirculatory network of soft tissues in patients with critical lower limb ischemia. The study was conducted in 210 patients (154 men, 56 women) with critical lower limb ischemia aged from 28 to 74 years, who were receiving hospital tre- atment during 2001-2019, with the ischemia duration of two-months-up-to-four-years. The cause of critical ischemia was the occlusion of the arteries of the femoral-popliteal-tibia and tibia-foot seg- ments of the atherosclerotic and thromboangiotic genesis. The regional blood flow was examined through non-invasive instrumental techniques (the determination of oxygen saturation of skin, rheovasography, Doppler ultrasonography, ultrasound duplex scanning). The state of the microvas- culature and neoangiogenesis was evaluated by complex morphological and immunohistochemical studies of tru-cut biopsy specimens of ischemic limb soft tissues in 80 patients (55 men, 25 women). Non-parametric tests were used to analyze digital data. It was found that in contrast to controls, the combined administration of intravenous laser blood irradiation and cytokine therapy with 'Roncoleukin' in the perioperative period of revascularizing osteotrepanation with intraosseous la- ser irradiation accelerates neoangiogenesis and increases the number of functionally active micro- vessels with open lumen in the soft tissues of ischemic limb. The statistically significant improve- ment in regional blood flow and skin oxygen saturation was observed after indirect revascularizati- on as well. The combination of intravenous laser blood irradiation and cytokine therapy in the pe- rioperative period of indirect revascularization by osteotrepanation with bone marrow laser irra- diation creates favorable conditions for increasing regional blood flow and promoting neoangioge- nesis to improve microvasculature in critically ischemic lower limb.
Keywords: Occlusion of arteries, critical ischemia, indirect revascularization, laser irradiation, cytokine therapy, regional hemodynamics, microvasculature
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