Engagement of mechanisms of cellular differentiation in formation of memory traces
Research article: Engagement of mechanisms of cellular differentiation in formation of memory traces
Authors: A.A. Mekhtiev1*, Sh.M. Asadova1, Sh.B. Guseinov1, G.R. Vagabova2
1Academician A.Garayev Institute of Physiology, Azerbaijan National Academy of Sciences, 78 Sharifzadeh, Baku AZ1100, Azerbaijan
2Azerbaijan State Medical University, 23 A.A.Bakikhanov, Baku AZ1022, Azerbaijan
*For correspondence: arifmekht@yahoo.com
Accepted for publication: 01 October 2019
Abstract:
The article concerns study of the effect of antibody-mediated blockade of serotonin-modulating an-ticonsolidation protein (SMAP), being in linear relation with serotonin, on the formation of me-mory in the rats on the conditioned models of alternative running and 2-lever operant differentiati-on with food reinforcement, as well as on the level of nerve growth factor (NGF) in the brain struc-tures. In the 1st series of studies in the rats, achieved 80% level of correct trials on the model of al-ternative running, through ELISA-test the level of SMAP was evaluated in the brain occipital and temporal cortex. Significant downregulation (p<0.001) of SMAP in the temporal cortex of the trai-ned rats was noticed. In the 2nd and 3rd series of studies a single intra-cerebral administration of the anti-SMAP antibodies prior to learning sessions brought to much quicker (p<0.001) formation of the memory (50% level of correct trials), than in the intact and control (non-immune γ-globulins) animals, as well as to a significantly quicker decrease of latency of the first running towards the platform or lever (p<0.01). In the 4th series of studies 24 h later since intra-cerebral administration of anti-SMAP antibodies induced downregulation of NGF in the hippocampus (p<0.001) and left parietal cortex (p<0.001), whereas 3 days later downregulation of NGF in the left parietal cortex (p<0.001) and its upregulation in the hippocampus (p<0.001) were noticed. It is proposed that, pro-moting effects of antibodies-mediated blockade of SMAP on the formation of memory traces are re-lated to its negative regulation of cellular differentiation.
Keywords: Serotonin-modulating anticonsolidation protein, antibodies, memory, indirect ELISA-test, nerve growth factor, hippocampus, left parietal cortex
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