Optimization of culture conditions for higher production of antimicrobial compounds by AZ-130 bacterial strain isolated from soil of Azerbaijan
Research article:Optimization of culture conditions for higher production of antimicrobial compounds by AZ-130 bacterial strain isolated from soil of Azerbaijan
Author: A.G. Aghayeva
Institute of Molecular Biology and Biotechnologies, Azerbaijan National Academy of Sciences, 11 Izzat Nabiyev, Baku AZ1122, Azerbaijan; For correspondence: aytanaghayeva@gmail.com
Accepted for publication: 08 November 2019
Abstract: AZ-130 bacterial strain was isolated from soil sample collected from Azerbaijan in 2014. After pre-liminarily culture and supernatant screenings for novel antibacterial compounds, AZ-130 showed strong gram-positive activity against pathogenic Staphylococcus aureus and Enterococcus faecalis strains. Based on range of its activity, AZ-130 strain that produces AZ-130 antibacterial compound was selected for the further characterization. The main goal of this study is to optimize growth con-ditions for AZ-130 to determine the optimal medium, incubation temperature and time point in which the production of the antimicrobial compound is highest. To achieve this goal, 4 different me-dia types at 4 different temperatures, in total 16 different growth conditions were tested. Superna-tants were collected and clarified at day 1, 2 and 3 or 5. All collected supernatants were analyzed by spot test and broth microdilution method against S.aureus. According to the spot test and broth mic-rodilution results, AZ-130 produces the most antimicrobial compound in TB + 2% Glucose medium at 32°C; incubation time is 2 days.
Keywords: Antimicrobial activity, antibiotics, bioactive molecules, culture conditions, medium optimiza-tion, natural products, primary metabolite, secondary metabolite, pathogenic bacteria
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