Impact of illumination on plant Photosystem II at different temperatures
Research article: Impact of illumination on plant Photosystem II at different temperatures
Author (s): A.M. Hasanova1, Y.M. Feyziyev1,2,*
1Institute of Molecular Biology and Biotechnologies, Azerbaijan National Academy of Sciences, 11 Izzat
Nabiyev Str., Baku AZ1073, Azerbaijan
2Research Institute of Crop Husbandry, Ministry of Agriculture of the Republic of Azerbaijan, Pirshaghy
Settlement, Sovkhoz No 2, Baku AZ 1098, Azerbaijan
*For correspondence: ya_feyziyev@yahoo.com
Received: October 22, 2020; Received in revised form: June 12, 2021; Accepted: June 18, 2021
https://doi.org/10.29228/jlsb.13
Journal of Life Sciences & Biomedicine, vol. 3(76), No 1, p. 99-107 (2021)
Abstract
The photochemical activity of photosystem II (PSII) illuminated at different temperatures (20-55℃) has been studied. The photochemical activity of PSII preparations incubated at different temperatures in the dark decreased sharply in the range of 40-55°C, and was relatively stable at temperatures 20-40°C. The photochemical activity of PSII decreased with relatively monotonous kinetics in preparations processed at different temperatures and exposed to light, but in the end, it was higher than the activity observed in samples incubated in the dark. The effects of glycerin and sucrose on the PSII inhibition due to the exposure to different temperatures in darkness, and after temperature-light treatment were studied. The photochemical activity of PSII was measured after the incubation of the samples in a solution containing 50% glycerol (volume) or 1 M sucrose for 5 minutes in the dark at a certain temperature or under high light intensity at the same temperature. The photochemical activity
of the PSII complex was found to be partially preserved in samples incubated in a glycerincontaining solution, at high temperatures, in both dark and light. The addition of sucrose to the solution resulted in a higher degree of protection of the photochemical activity of PSII. Keywords: Photosystem II, temperature, light, inhibition
Abbrevıatıons: BBY ((Berthold, Babcock, Yocum) Thylakoid membrane fragments enriched with PSII), Cyt b559 (Cytochrome b559), PSII (Photosystem II), FV (F0) (Variable (initial) fluorescence of chlorophyll), QA, QB (Plastoquinones, electron acceptors), MES (4-Morpholinye-ethane-sulfonic acid), organic buffer), P680 (Primary donor of the electron in PSII), Pheo (Pheophytin), RC (Reaction center), YZ, YD (Tyrozines, electron donors of P680)
Author (s): A.M. Hasanova1, Y.M. Feyziyev1,2,*
1Institute of Molecular Biology and Biotechnologies, Azerbaijan National Academy of Sciences, 11 Izzat
Nabiyev Str., Baku AZ1073, Azerbaijan
2Research Institute of Crop Husbandry, Ministry of Agriculture of the Republic of Azerbaijan, Pirshaghy
Settlement, Sovkhoz No 2, Baku AZ 1098, Azerbaijan
*For correspondence: ya_feyziyev@yahoo.com
Received: October 22, 2020; Received in revised form: June 12, 2021; Accepted: June 18, 2021
https://doi.org/10.29228/jlsb.13
Journal of Life Sciences & Biomedicine, vol. 3(76), No 1, p. 99-107 (2021)
Abstract
The photochemical activity of photosystem II (PSII) illuminated at different temperatures (20-55℃) has been studied. The photochemical activity of PSII preparations incubated at different temperatures in the dark decreased sharply in the range of 40-55°C, and was relatively stable at temperatures 20-40°C. The photochemical activity of PSII decreased with relatively monotonous kinetics in preparations processed at different temperatures and exposed to light, but in the end, it was higher than the activity observed in samples incubated in the dark. The effects of glycerin and sucrose on the PSII inhibition due to the exposure to different temperatures in darkness, and after temperature-light treatment were studied. The photochemical activity of PSII was measured after the incubation of the samples in a solution containing 50% glycerol (volume) or 1 M sucrose for 5 minutes in the dark at a certain temperature or under high light intensity at the same temperature. The photochemical activity
of the PSII complex was found to be partially preserved in samples incubated in a glycerincontaining solution, at high temperatures, in both dark and light. The addition of sucrose to the solution resulted in a higher degree of protection of the photochemical activity of PSII. Keywords: Photosystem II, temperature, light, inhibition
Abbrevıatıons: BBY ((Berthold, Babcock, Yocum) Thylakoid membrane fragments enriched with PSII), Cyt b559 (Cytochrome b559), PSII (Photosystem II), FV (F0) (Variable (initial) fluorescence of chlorophyll), QA, QB (Plastoquinones, electron acceptors), MES (4-Morpholinye-ethane-sulfonic acid), organic buffer), P680 (Primary donor of the electron in PSII), Pheo (Pheophytin), RC (Reaction center), YZ, YD (Tyrozines, electron donors of P680)
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